Use a clean cage when combining males from different litters to prevent territorial behavior. The IACUC acknowledges that some specialized genetically modified lines may require more than 2 adults and 1 litter in the breeding cage to facilitate adequate production. <> The primary objective of this code is to establish minimum standards and promote recommendations for the housing and care of … GUIDELINES FOR RATS AND MICE . Title: Guidelines for the Housing of Rats in Scientific Institutions Author: NSW Department of Primary Industries Created Date: 1/24/2008 1:20:25 PM Investigators must submit a BUworks Internal Service Request (ISR) for per diems and for special service charges prior to placing animal orders. Whilst we appreciate the efforts made by individuals to improve the living conditions of those animals unfortunate enough to be used in research, AAHR feels it would be inappropriate to Many experimental approaches require housing rodents in individual cages, including in epilepsy research. It is suggested that the housing of laboratory rodents … Housing for Laboratory Rats, Mice, Guinea pigs and Rabbits. HOUSING AND CARE preprint issued by THE ROYAL SOCIETY 6 Carlton House Terrace, London SWlY SAG and UNIVERSITIES FEDERATION FOR ANIMAL WELFARE 8 Hamilton Close, South … stream Fighting among adult male mice is a well-documented behavior and can result in severe wounding and death. To minimize fighting, the following group-housing practices are followed for mice: Do not combine adult male mice (older than 5 weeks). The NACLAR Guidelines set out the responsibilities of The National Research Council (NRC) “Guide for the Care and Use of Laboratory Animals” (2011) provides recommended minimum housing space expectations for laboratory rats and mice (p.57). Code of practice for the housing and care of. This review should begin no later than October 1, 2020. %���� Ventilated cages were designed to prevent the accumulation of NH Provides guidance on best practice standards for housing animals based on published scientific information and practices employed in New South Wales, Australia. These guidelines have been written to assist faculty, staff, and students in performing vertebrate animal procedures in a humane manner and complying with pertinent regulatory requirements. Code of Practice for the Housing and Care of Animals Bred, Supplied or Used for Scientific Purposes Contents 4.15 Specific requirements of breeding animals 79 Section 3, Chapter 2: Mice, rats, gerbils, hamsters and guinea pigs 80 1 Advice applicable to all rodents 80 1.1 Environmental conditions 80 1.2 Health 81 1.3 Housing, enrichment and care 81 8,9 Indeed, many studies have compared individual versus group housing of rodents under laboratory conditions. Authorization for Animal Housing and Special Services Costs. Tumour Housing for Laboratory Rats, Mice, Guinea pigs and Rabbits. in. x��Zmo�� ���'X+�(�X�$�w[����i��[���Z>EN����BR�m�B�=G���yy晡��o����_n?݉��Z��݊��db�ry���E�đ1�OQ��,uTj�e2�Z,�]^\}�V�k-������[!�����(�2���,U�Tf�LYb*�Ab,��Ϗ�����?�����O�NGq�t)���,_����/�ٮ�����|���~l�5Ic\Px��be��@�jjD�4��b��tDHRFI6E�"�$$���ڮŬ�.��e>"1�"��w`�t&~���v;_���j����m.�l9_�Y��ŬCf��H�P��Z��.ӨH�����'�>��z��b.��_������#�T����Qm���!��n�4+���}�'j��L���}�I�(K���m��>�P��䈛�"�5�)⥋��m������}P�1?��?���Ͽ����{�7:�@"a�,Gl��P�h���U���(�JT��H=�XN���(/�H��T�2��|�H9Ud�D��$2�(2-e�؈���V �@��$>�`wݻA� Forty-one percent of institutions reported housing 0-20% of their mice under conventional conditions. Mouse rotavirus (MRV) is a group A rotavirus that replicates in the villus epithelial cells of the small intestine. Guide to the Care and Use of Experimental Animals, Volume 1 and 2, Canadian Council on Animal Care. <>>> Ann Arbor, MI 48109 Deviation from Guide should statement according to a locally established performance standard. An exception to a Policy must be described and justified in the Animal Protocol and approved by the full IACUC at a convened monthly meeting. %PDF-1.5 • If scientifically justified in an approved IACUC protocol, mice may be individually housed in specific situations outlined in the IACUC protocol. The NACLAR Guidelines set out the responsibilities of all the parties involved in the care and use of animals for scientific purposes, in accordance with widely accepted scientific, ethical and legal principles. Email: acuoffice@umich.edu, HUSBANDRY & VETERINARY CARE Housing and caretaking of the mice at the K.U.Leuven partner is in accordance with the Belgian and European laws, guidelines and policies for animal experimentation, housing and care (as documented in the most recent legislation, i.e. Some colleagues have recommended housing intact male mice in groups of 3 to maintain stable dominance hierarchies and minimize aggression. With a physical land area of just over 700 square kilometers and a population of around 5.4 million people, this densely populated island has abundant tropical flora, parks, and g… Many murine pathogens are known to result in immune perturbations that can affect research outcomes (Hsu, et al., 2016; Macy, et al., 2011). Guidelines for the housing of mice in scientific institutions (PDF|3.8 MB) Guidelines for the housing of guinea pigs in scientific institutions … There is wide agreement in the scientific community that advances in laboratory animal health have enhanced the reliability of animal-based research. No. the draft guidelines for the housing of mice in scientific institutions. The National Research Council (NRC) “Guide for the Care and Use of Laboratory Animals” (2011) provides recommended minimum housing space expectations for laboratory rats and mice (p.57). is recommended for principles of rabbit pen design. The design of animal housing should facilitate well being of the animals, meet research requirements, minimise experimental variables, and isolate the animals from wide variations in temperature and humidity and from vibration and sources of loud noise. the Belgian Royal Decree of 6 April 2010 and European Directive 2010/63/EU on the protection of animals used for scientific purposes of 20 October 2010). Effect of the Extract on the Intestinal Transit of Charcoal Meal in Mice. Canadian Council on Animal Care. Current guidelines recommend housing mice in social groups as they are highly social animals, however, aggression and fighting can occur between group-housed males leading to injuries and potentially death. no extended weaning is allowed if post-partum estrus is used. <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 28, 29 Using our strategy, we have been able to group-house as many as 10 castrated mice, in a large rat cage with enrichment materials, in harmonious social conditions. Based on data from Guidelines for the housing of Mice in Scientific Institutions, 2012, the consumption of mice feed range from 4-8 grams/day and water consumption 5-8 ml/day (Fawcett, 2013). 2 0 obj The Code of Practice for Housing and Care of Laboratory Mice, Rats, Guinea Pigs and Rabbits applies throughout Victoria. Q�N���e9k��DjϋY�O��� �!�=� Guidelines for the housing of mice in scientific institutions (PDF|3.8 MB) Guidelines for the housing of guinea pigs in scientific institutions (PDF|309.3 KB) Nursing mothers or breeding pairs are kept in a quiet cage or tub with no wire floor. To minimize fighting, the following group-housing practices are followed for mice: Do not combine adult male mice (older than 5 weeks). The normal total cholesterol level is 26.0-82.4 mg/dl. National Academy of Sciences is a private, nonprofit, self-perpetuating society of distinguished scholars engaged in scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare. Both cases require work in higher biosafety level containments, additional screening methods and special guidelines for the handling and restraint of such animals. For infectious experiments, mice are transferred to a designated biosafety level 2 facility and usually are … Limiting breeding cage occupancy reduces the incidence of morbidity and mortality due to overcrowding. The 'Guidelines for the Housing of Rabbits in Scientific Institutions ' (see Section 4.) For infectious experiments, mice are transferred to a designated biosafety level 2 facility and usually are housed in static cages for the study duration. Guidelines for Transgenic Research. Basic requirements for good rodent housing and husbandry are given in the table below. Enclosures designed to cause minimum disturbance to the animals. Guidelines for the Housing of Mice in Scientific Institutions Table of Contents RECOMMENDATIONS 4 1.0 GENERAL 20 1.1 INTRODUCTION 20 1.2 RESPONSIBILITIES OF INSTITUTIONS 21 1.3 RESPONSIBILITIES OF CHIEF INVESTIGATORS/ TEACHERS 21 1.4 THE AUSTRALIAN CODE OF PRACTICE FOR THE According to UK Home Office guidelines, the room temperature for mice should be 20–24°C (68–75°F) and kept as stable as possible . Management of Fighting Mice . Unit for Laboratory Animal Medicine (ULAM) This does not apply to: Littermates that have been cage mates since birth. Generally, in light of mice being gregarious animals, it is recommended to avoid individual housing whenever possible. Code of Practice for the Housing and Care of Laboratory Mice Rats Guinea Pigs. Ann Arbor, MI 48109 These rodents are social species and should be housed together, unless scientifically justified for individual housing. Housing of Social Species (Policy) Policy: The IACUC has provided a set of guidance documents (Policies, Guidelines, and Informational Sheets) for use when planning animal procedures at the University of Iowa. if housed in standard #3 rat caging. Some strains are more aggressive than others, and fight regardless of the age at combination. GUIDANCE & OVERSIGHT We have reviewed 40 studies carried out between 1987 and 2000, in which preferences as well as the effect of housing modifications have been studied. It is questionable whether conventional housing systems for rodents such as mice, rats and guinea-pigs meet this demand. Phone: (734) 764-0277 11 However, the observed effects can depend on the sex and strain of the animals on the one hand, and on the cage and laboratory environment on the other hand. Ph (02) 6391 3682 Fax (02) 6391 3570 or Sydney Office Ph (02) 9872 0570 Fax (02) 9871 6938. These breeding strategies are only allowable provided that pregnant females are separated prior to parturition and only 1 litter of pups with up to 2 adults remain in the cage after pups are born. While we have successfully managed many mouse colonies over the years using this system, we are uncertain that the cage space guidelines described in the 8th edition of the Guide allows trio-breeding schemes in standard cages. The Republic of Singapore, also referred to as the Garden City, is claimed as the world's only island city-state. Code of Practice for the Housing and Care of Animals Bred, Supplied or Used for Scientific Purposes Contents 4.15 Specific requirements of breeding animals 79 Section 3, Chapter 2: Mice, rats, gerbils, hamsters and guinea pigs 80 1 Advice applicable to all rodents 80 1.1 Environmental conditions 80 1.2 Health 81 1.3 Housing, enrichment and care 81 The . Housing and caretaking of the mice at the K.U.Leuven partner is in accordance with the Belgian and European laws, guidelines and policies for animal experimentation, housing and care (as documented in the most recent legislation, i.e. This does not apply to: Littermates that have been cage mates since birth. This preview shows page 45 - 48 out of 83 pages. No, although OLAW encourages institutions to implement the new guidelines as soon as possible, the review of already approved protocols can be phased in as part of the review processes already in place at Assured institutions. 3 0 obj Although a strain is considered stable when backcrossed for 10 generations, original 129 genes that are too close to the mutated gene to be segregated by … There is no doubt that the housing conditions have a robust influence on the behavior and physiology of laboratory mice. Delayed weaning (weaning at >21 days) requires prior IACUC approval. Typical husbandry conditions in our institution allow housing a maximum of 5 mice per cage and changing bedding every 2 wk within an SPF barrier facility. Therefore, it is unclear why housing many laboratory mice (>4), instead of one-or-two per cage, with the improper use/reporting of clustered-data statistics, abound in the literature. Purpose. House males individually in these circumstances, or with females, if intending to breed. 3.5. OLAW FAQ G10, Guide pp 6-7. (Enclosures should allow for a minimum floor area of 250cm2 for a single housed mouse, a minimum floor area of 500cm2 for two mice and ensuring a minimum floor area of 60cm2 per additional adult mouse when mice are housed in larger groups.) … CCAC guidelines: Mice 1 PREFACE The Canadian Council on Animal Care (CCAC) is the national peer-review organization responsible for setting and maintaining standards for the ethics and care of animals in science throughout Canada. Find the maximum number of rats and mice that can be house in each cage in the Rodent Cage Capacity Policy. If post-partum estrus is used in "standard" mouse cages, the first litter must be weaned by 21 days of age to prevent the presence of two litters in a cage, i.e. Fighting among adult male mice is a well-documented behavior and can result in severe wounding and death. endobj The NC3Rs have led a data crowdsourcing project, which estimated the prevalence of aggression in UK facilities as approximately 15 in every 1,000 mice. Abstract. Canadian Council on Animal Care. In all cases, cages should be regularly monitored to ensure the well-being of the neonates (e.g., size, age, and activity level of litter), as well as characteristics of the cage environment and colony breeding performance. endobj Guidelines for the Housing of Rabbits in Scientific Institutions, NSW Animal Research Review Panel. The authors concluded that housing mice at twice Guide-recommended densities has no deleterious impact on any of the parameters assessed. Code of Practice for the Housing and Care of Animals Bred, Supplied or Used for Scientific Purposes Contents 4.15 Specific requirements of breeding animals 79 Section 3, Chapter 2: Mice, rats, gerbils, hamsters and guinea pigs 80 1 Advice applicable to all rodents 80 1.1 Environmental conditions 80 1.2 Health 81 1.3 Housing, enrichment and care 81 Guideline No. Whilst we appreciate the efforts made by individuals to improve the living conditions of those animals unfortunate enough to be used in research, AAHR feels it would be inappropriate to comment on a system that we consider to be scientifically flawed and are fundamentally opposed to. Guidelines for the Housing of Rabbits in Scientific Institutions, NSW Animal Research Review Panel. Mild to moderate wounds (those involving minimal damage to the epidermis) necessitates consultation with ULAM veterinary personnel for determination of appropriate therapy. <> However, rats and mice are social animals; and individual housing constitutes a stressful situation. Guidelines for Housing Capacity. To ensure reproducibility and reduce variability, institutions have moved toward housing mice in barrier facilities and maintaining colonies in a specific pathogen-free (SPF) status. New arrival mice. The most common breeding scheme for mice at our AAALAC accredited institution is trio-breeding. Housing in stable, compatible groups - it is important to take into account sex, age, reproductive condition, familiarity, prior group housing experience when grouping the animals. 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